Cyanine5.5 phosphoramidite
Artikel-Nr. | Packungseinheit | Preis | Vorlaufzeit | Jetzt kaufen |
---|---|---|---|---|
2680-100mg
/ in einer 6-ml-Septumflasche
|
100 mg | – | 5 Tagen. | |
2680-250mg
/ in einer 6-ml-Septumflasche
|
250 mg | $290 | Auf Lager | |
2680-1g
/ in einer 6-ml-Septumflasche
|
1 g |
$950
|
Auf Lager | |
2680-10g
/ in einer 30-ml-Septumflasche
|
10 g | auf Anfrage | Auf Lager |
Cyanine5.5 is a fluorophore with an emission in the far-red range of the spectrum. This dye is useful for multiplex qPCR. Commercial six-channel qPCR instruments often have a channel for Cyanine5.5.
This phosphoramidite can be used for the synthesis of 5'-labeled oligonucleotides by direct labeling in an oligonucleotide synthesizer.
The structure of the phosphoramidite functional group that is attached to a secondary carbon atom provides extra stability against Arbuzov rearrangement. This helps maintain coupling performance over a longer storage time in oligonucleotide synthesizers, compared to phosphoramidites derived from primary alcohols.

Kunden kauften zusammen mit diesem Produkt
ROX NHS-Ester, 6-Isomer
Reines 6-Isomer des ROX (Rhodamin-X, R101) NHS-Esters für Aminmarkierung.Cyanin3.5-Azid
Cyanin3.5-Azid für die Markierung von Biomolekülen mittels Click-Chemie.DusQ® 1 CPG 500
Ein CPG 500-Trägermaterial zur Synthese von Oligonukleotiden mit DusQ® 1-Quencher am 3'-Ende. Es ist mit Standardbedingungen zum Entschützen von Oligonukleotiden kompatibel.General properties
Appearance: | dark colored solid |
Molecular weight: | 916.61 |
Molecular formula: | C55H71N5ClO3P |
Quality control: | NMR 1H, 31P, HPLC-MS (85%) |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Sicherheitsdatenblatt: | herunterladen |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 694 |
ε, L⋅mol−1⋅cm−1: | 198000 |
Emission maximum, nm: | 710 |
Fluorescence quantum yield: | 0.2 |
CF260: | 0.07 |
CF280: | 0.03 |
Oligo synthesis details
Diluent: | acetonitrile |
Coupling conditions: | 6 min coupling time recommended |
Deprotection conditions: | recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible |
