Cyanine5.5 phosphoramidite
Artikel-Nr. | Packungseinheit | Preis | Vorlaufzeit | Jetzt kaufen |
---|---|---|---|---|
2680-100mg | 100 mg | – | Auf Lager | |
2680-250mg | 250 mg | $290 | Auf Lager | |
2680-1g | 1 g | $950 | Auf Lager | |
2680-10g | 10 g | auf Anfrage | 5 Tagen. |
Cyanine5.5 is a fluorophore with the emission in far-red range of the spectrum. This dye is useful for multiplex qPCR. Commercial six-channel qPCR instruments often have a channel for Cyanine5.5.
This phosphoramidite can be used for the synthesis of 5'-labeled oligonucleotides by direct labeling in oligonucleotide synthesizer.
The structure of phosphoramidite functional group that is attached to a secondary carbon atom provides extra stability against Arbuzov rearrangement. This helps maintaining coupling performance over a longer storage time in oligonucleotide synthesizer, compared to phosphoramidites derived of primary alcohols.
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DusQ 3 CPG 500
DusQ 3 ist ein dunkler Quencher für Farbstoffe im fernen Rot und im nahen Infrarot wie Cyanin5.5. Dies ist ein fester Glasträger mit kontrollierten Poren für die Synthese von Oligonukleotiden, wobei der Quencher am 3'-Terminus angebracht ist.ROX NHS-Ester, 6-Isomer
Reines 6-Isomer des ROX (Rhodamin-X, R101) NHS-Esters für Aminmarkierung.Inosin (dI) Phosphoramidit
Dimethoxytrityl-2'-Desoxyinosin (DMT-dI) ist ein Phosphoramidit für die Synthese inosinhaltiger DNA-Oligonukleotide.General properties
Appearance: | dark colored solid |
Molecular weight: | 916.61 |
Molecular formula: | C55H71N5ClO3P |
Quality control: | NMR 1H, 31P, HPLC-MS (85%) |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Sicherheitsdatenblatt: | herunterladen |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 694 |
ε, L⋅mol−1⋅cm−1: | 198000 |
Emission maximum, nm: | 710 |
Fluorescence quantum yield: | 0.2 |
CF260: | 0.07 |
CF280: | 0.03 |
Oligo synthesis details
Diluent: | acetonitrile |
Coupling conditions: | 6 min coupling time recommended |
Deprotection conditions: | recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible |