Einige Inhalte auf dieser Seite sind in deutscher Sprache nicht verfügbar und werden automatisch in derzeit verfügbarer Sprache angezeigt.

Cyanine5 phosphoramidite

Cyanin 5 Phosphoramidite
Artikel-Nr. Packungseinheit Preis Vorlaufzeit
1740-1g 1 g $690.00 21 Tagen.
1740-10g 10 g auf Anfrage 21 Tagen.
Großmengen-Anfrage

Cyanine5 is one of the dyes that are used in qPCR. The corresponding filter set is found on many qPCR machines.

This reactive derivative is useful for the synthesis of qPCR probes having Cyanine5 at 5'-end, the most typical location of fluorophore. This is a terminating, non-nucleoside reagent.

Amidite group in this reagent is bound to a secondary oxygen. This provides extra stability against Arbuzov rearrangement - a reaction that leads to amidite deterioration in solution in oligonucleotide synthesizer. This molecular design increases stability of the reagent, maintaining efficient coupling over longer time.

Herunterladen im
ChemDraw-Format

General properties

Appearance: dark colored solid
Molecular weight: 816.49
Molecular formula: C47H67N5ClO3P
Quality control: NMR 1H, 31P, HPLC-MS (85%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
Sicherheitsdatenblatt:: herunterladen
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: 6 min coupling time recommended
Deprotection conditions: recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible
Sie haben den Artikel in den Warenkorb gelegt.. Warenkorb ansehen oder zur Kasse gehen
Die eingegebene Zahl ist falsch..