Cyanine5 phosphoramidite
Artikel-Nr. | Packungseinheit | Preis | Vorlaufzeit | Jetzt kaufen |
---|---|---|---|---|
1740-100mg | 100 mg | – | Auf Lager | |
1740-250mg | 250 mg | $290 | Auf Lager | |
1740-1g | 1 g |
$950
|
Auf Lager | |
1740-10g | 10 g |
$6750
|
Auf Lager |
Cyanine5 is one of the dyes that are used in qPCR. The corresponding filter set is found on many qPCR machines.
This reactive derivative is useful for the synthesis of qPCR probes having Cyanine5 at 5'-end, the most typical location of the fluorophore. This is a terminating, non-nucleoside reagent.
Amidite group in this reagent is bound to a secondary oxygen. This provides extra stability against Arbuzov rearrangement - a reaction that leads to amidite deterioration in solution in oligonucleotide synthesizer. This molecular design increases the stability of the reagent, maintaining efficient coupling over a longer time.
![](https://static.lumiprobe.us/img/spectra/Cy5_absorption_emission.png)
Kunden kauften zusammen mit diesem Produkt
FAM-dT-Phosphoramidit
Phosphoramidit für die Synthese von Oligonukleotiden mit interner FAM-Modifikation.DMS(O)MT Aminolink C6
Ein Amidit für die Einführung einer Aminogruppe für die Oligonukleotidsynthese.DusQ 3 CPG 500
DusQ 3 ist ein dunkler Quencher für Farbstoffe im fernen Rot und im nahen Infrarot wie Cyanin5.5. Dies ist ein fester Glasträger mit kontrollierten Poren für die Synthese von Oligonukleotiden, wobei der Quencher am 3'-Terminus angebracht ist.General properties
Appearance: | dark colored solid |
Molecular weight: | 816.49 |
Molecular formula: | C47H67N5ClO3P |
Quality control: | NMR 1H, 31P, HPLC-MS (80%) |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Sicherheitsdatenblatt: | herunterladen |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 646 |
ε, L⋅mol−1⋅cm−1: | 250000 |
Emission maximum, nm: | 662 |
Fluorescence quantum yield: | 0.2 |
CF260: | 0.03 |
CF280: | 0.04 |
Oligo synthesis details
Diluent: | acetonitrile |
Coupling conditions: | 6 min coupling time recommended |
Deprotection conditions: | recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible |
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