Alkyne dT phosphoramidite
Artikel-Nr. | Packungseinheit | Preis | Vorlaufzeit | Jetzt kaufen |
---|---|---|---|---|
1897-100mg | 100 mg | $330 $195 | Auf Lager | |
1897-1g | 1 g | $2590 $1250 | Auf Lager | |
1897-5g | 5 g | $9900 $2850 | Auf Lager |
This reagent is designed for the synthesis of oligonucleotides bearing triple bond (alkyne) in the middle of the chain. Alkyne is attached to the deoxyuridine fragment, and it does not interfere with the hybridization of the complementary strand. This allows the preparation of modified oligonucleotide probes bearing fluorescent dyes, quenchers, and other fragments attached to the middle of the chain by virtue of copper-catalyzed Click chemistry reaction with various azides.
This phosphoramidite is solid, so it is easy to dispense and handle. It is compatible with the standard deblocking conditions.
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DusQ 1-Phosphoramidit, 5'-Terminal
Es ist ein terminales Phosphoramidit zur Markierung von Oligonukleotiden am 5'-Ende mit DusQ 1-Quencher.Inosin (dI) Phosphoramidit
Dimethoxytrityl-2'-Desoxyinosin (DMT-dI) ist ein Phosphoramidit für die Synthese inosinhaltiger DNA-Oligonukleotide.Phosphat-CPG-Träger 500
CPG-Trägermaterial für die Synthese 3'-phosphorylierter Oligonukleotide.General properties
Appearance: | off white solid |
Mass spec M+ increment: | 437.1 |
Molecular weight: | 877.96 |
Molecular formula: | C48H56N5O9P |
Solubility: | good in acetonitrile, DCM |
Quality control: | NMR 1H, NMR 31P, HPLC-MS |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
Sicherheitsdatenblatt: | herunterladen |
Product specifications |
Oligo synthesis details
Diluent: | acetonitrile |
Coupling conditions: | standard coupling, identical to normal nucleobases |
Cleavage conditions: | ammonia, 2 h at room temperature |
Deprotection conditions: | identical to protected nucleobases |