Cyanine3 phosphoramidite 5'
|2771-100mg||100 mg||–||Auf Lager|
|2771-250mg||250 mg||$290.00||Auf Lager|
|2771-1g||1 g||$950.00||Auf Lager|
|2771-10g||10 g||$6750.00||21 Tagen.|
Cyanine3 is a fluorophore that is widely used in molecular biology experiments such as oligonucleotide labeling followed by oligonucleotide detection. By its spectral characteristics, Cyanine3 is an analog of Cy™3 with a fluorescence maximum at 570 nm in the yellow spectrum range.
Cyanine3 phosphoramidite 5’ is used in oligonucleotide synthesis for the production of 5’-cyanine3-labeled oligonucleotides. The reagent is compatible with various oligonucleotide synthesizers.
This phosphoramidite can be used for the synthesis of fluorescence-labeled primers and hybridization probes such as TaqMan and Molecular Beacon. Such labeled probes can be detected in multiplex real-time PCR in the TAMRA channel.
Condensation: 3 min. Use 0.02 M iodine solution at the oxidation step to avoid degradation of the cyanine dye.
Deprotection: At room temperature with 30% aqueous ammonium solution. It is recommended to use nucleic bases with labile protective groups for deprotection for not more than 2 h at less than 55°C. AMA (30% aqueous ammonium solution/40% aqueous methylamine 1:1 (v/v)) can be used for 10 min at 65°C in the presence of acetyldeoxycytidine. If deoxyguanidine with a dimethylformamidine protective group is used during synthesis, deprotect with 30% aqueous ammonium solution for 2 h at 65°C. If deoxyguanidine with an isobutyryl protective group is used during synthesis, deprotect for 24-36 h at room temperature.
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|Solubility:||good in acetonitrile, dichloromethane|
|Quality control:||NMR 1H, 31P, HPLC-MS (80%)|
|Storage conditions:||Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.|
|Excitation/absorption maximum, nm:||555|
|Emission maximum, nm:||570|
|Fluorescence quantum yield:||0.31|
Oligo synthesis details
|Coupling conditions:||6 min coupling time recommended|
|Deprotection conditions:||recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible|