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Cyanine5 phosphoramidite
| Artikel-Nr. | Packungseinheit | Preis | Vorlaufzeit | Jetzt kaufen |
|---|---|---|---|---|
| 1740-100mg | 100 mg | – | Auf Lager | |
| 1740-250mg | 250 mg | $290 | Auf Lager | |
| 1740-1g | 1 g |
$950
|
Auf Lager | |
| 1740-10g | 10 g |
$6750
|
Auf Lager |
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Cyanine5 is one of the dyes that are used in qPCR. The corresponding filter set is found on many qPCR machines.
This reactive derivative is useful for the synthesis of qPCR probes having Cyanine5 at 5'-end, the most typical location of the fluorophore. This is a terminating, non-nucleoside reagent.
Amidite group in this reagent is bound to a secondary oxygen. This provides extra stability against Arbuzov rearrangement - a reaction that leads to amidite deterioration in solution in oligonucleotide synthesizer. This molecular design increases the stability of the reagent, maintaining efficient coupling over a longer time.
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TAMRA-Phosphoramidit, 6-Isomer
TAMRA-Phosphoramidit für die Synthese 5'-markierter Oligonukleotide, reines 6-Isomer.JOE phosphoramidite, 5-isomer
JOE phosphoramidite is a xanthene dye phosphoramidite used in oligo synthesis. Its absorption and emission spectra are located between the FAM and TAMRA channels.Cyanin3 NHS-Ester
Cyanin3-Farbstoff NHS-Ester für die Markierung von Aminogruppen in Peptiden, Proteinen und Oligonukleotiden.General properties
| Appearance: | dark colored solid |
| Molecular weight: | 816.49 |
| Molecular formula: | C47H67N5ClO3P |
| Quality control: | NMR 1H, 31P, HPLC-MS (80%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| Sicherheitsdatenblatt: | herunterladen |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | 6 min coupling time recommended |
| Deprotection conditions: | recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible |
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