Cyanine3 CPG 1000
| Artikel-Nr. | Packungseinheit | Preis | Vorlaufzeit | Jetzt kaufen |
|---|---|---|---|---|
| 5377-100mg | 100 mg |
$156
|
21 Tagen. | |
| 5377-1g | 1 g |
$990
|
21 Tagen. |
Cyanine3 CPG 1000 is a controlled-pore glass (CPG) with a Cyanine3 dye covalently attached via a linker. The support is intended for the automated solid-phase synthesis of oligonucleotides using the phosphoramidite method. Its use allows for obtaining an oligonucleotide with a fluorescent label at the 3′-end without additional post-synthetic labeling steps.
The pore size of 1000 Å is optimal for synthesizing long oligonucleotide chains, up to 120 nucleotides in length. Cyanine3 (Cy3®) is a bright fluorescent dye in the orange-red spectral region with an absorption maximum at 555 nm and an emission maximum around 570 nm.
Oligonucleotides synthesized using this reagent can be used in molecular biology methods such as real-time PCR (qPCR), fluorescent in situ hybridization (FISH), FRET, and surface-enhanced Raman spectroscopy (SERS).
Absorptions- und Emissionsspektren von Cyanin3
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General properties
| Appearance: | pink beads |
| Quality control: | NMR 1H and loading measurement, functional testing in oligo synthesis. |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| Sicherheitsdatenblatt: | herunterladen |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 555 |
| ε, L⋅mol−1⋅cm−1: | 150000 |
| Emission maximum, nm: | 570 |
| Fluorescence quantum yield: | 0.31 |
| CF260: | 0.04 |
| CF280: | 0.09 |
Oligo synthesis details
| Pore size, Å: | 1000 |
| Typical loading, umol/g: | 25-40 |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Cleavage conditions: | ammonia, 2 h at room temperature. DO NOT use AMA mixture! |
| Deprotection conditions: | identical to protected nucleobases |
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