Di-4-ANEPPS, potentiometric probe
| Artikel-Nr. | Packungseinheit | Preis | Vorlaufzeit | Jetzt kaufen |
|---|---|---|---|---|
| 3429-50ug | 50 ug | – | Auf Lager | |
| 3429-5mg | 5 mg | $225 | Auf Lager |
Di-4-ANEPPS is an Amino-Naphthyl-Ethenyl-Pyridinium (ANEP) family voltage-sensitive dye widely used as a fast-responding membrane potential probe. The dye is non-fluorescent until bound to membranes and fluoresces only in response to electrical potential fluctuations in its environment.
The optical response of Di-4-ANEPPS is fast enough to detect transient (millisecond) potential changes in excitable cells, such as single neurons, cardiac cells, and intact brains. The magnitude of potential-dependent fluorescence change is about 2-10% per 100 mV. The dye also displays a potential-dependent shift in excitation spectrum, permitting the quantitation of cell membrane potential using ratiometric techniques.
Di-4-ANEPPS is quickly internalized by cells, so it is primarily used for short-term studies. We also provide Di-8-ANEPPS, which is more hydrophobic and better retained in the outer leaflet of the cell membrane. Since Di-4-ANEPPS binds to the cell membrane, it can also be used as a plasma membrane and endocytosis marker.
Excitation/emission maxima of Di-4-ANEPPS in methanol are 496/705 nm, respectively. In lipids and cell membranes, the excitation and emission spectra of the dye are typically blue-shifted compared to organic solvent.
Di-4-ANEPPS can be introduced into cells by directly adding the stock solution to the culture medium, using Pluronic® F-127, or retrograde labeling. Use a 5-10 μM working concentration as a starting point. The exact dye concentration should be defined experimentally.
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RH 237, potentiometrische Sonde
Schnell reagierende, potentialempfindliche fluoreszierende Sonde zur Bildgebung der funktionellen Aktivität von Mitochondrien, Neuronen und Herzzellen.Di-8-ANEPPS, potentiometric probe
Fast-response potential-sensitive fluorescent probe for imaging functional activity of neurons and heart cells. The dye has more lipophilic properties than other dyes of the ANEP family, making it well-suited for long-term experiments.General properties
| Appearance: | red solid |
| Molecular weight: | 480.67 |
| CAS number: | 90134-00-2 |
| Molecular formula: | C28H36N2O3S |
| Solubility: | ethanol, DMF, DMSO |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| Sicherheitsdatenblatt: | herunterladen |
| Product specifications |
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