Fura-2 AM, green fluorescent calcium indicator
| Artikel-Nr. | Packungseinheit | Preis | Vorlaufzeit | Jetzt kaufen |
|---|---|---|---|---|
| 4607-1mg | 1 mg |
$164.00
|
21 Tagen. |
Fura-2 AM is a classic ratiometric fluorescent indicator for calcium ions, used to monitor intracellular Ca2+ concentrations in living cells. Thanks to its AM-ester form, the dye readily permeates the cell membrane and, following hydrolysis by intracellular esterases, is retained within the cytoplasm. Fura-2 is characterized by a shift in its excitation spectrum upon binding calcium and is typically measured in a 340/380 nm ratiometric mode, with emission occurring at approximately 510 nm. This dye enables quantitative analysis of calcium signals and corrects for artifacts associated with non-uniform dye loading, cell thickness, and photobleaching.
Compared to Fluo-4 AM, Fura-2 AM provides more accurate and reproducible measurements of basal calcium levels and slow changes in Ca2+ concentration, thanks to its ratiometric approach. Conversely, Fluo-4 AM exhibits significantly higher brightness and is compatible with the standard 488 nm lasers found in confocal microscopes, making it the preferred choice for detecting rapid calcium transients and for high-throughput screening. Fura-2 AM is more frequently used in applications where quantitative determination of calcium concentration is critical, whereas Fluo-4 AM is better suited for highly sensitive visualization of dynamic signals.
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General properties
| Appearance: | lemon powder |
| Molecular weight: | 1001.86 |
| CAS number: | 108964-32-5 |
| Molecular formula: | C44H47N3O24 |
| Solubility: | good in DMSO, methylene, and acetonitrile; poor in alcohols |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| Sicherheitsdatenblatt: | herunterladen |
| Product specifications |
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