AF 594 C5 maleimide
| Artikel-Nr. | Packungseinheit | Preis | Vorlaufzeit | Jetzt kaufen |
|---|---|---|---|---|
| 17880 | 1 mg |
$179.00
|
12 Tagen. | |
| 27880 | 5 mg |
$599.00
|
12 Tagen. | |
| 47880 | 25 mg |
$1680.00
|
12 Tagen. | |
| 67880 | 100 mg |
$3049.00
|
12 Tagen. |
AF594 C5 Maleimide is a bright, photostable fluorescent dye functionalized with a maleimide group via a flexible pentamethylene (C5) spacer. The maleimide moiety reacts selectively and rapidly with sulfhydryl (—SH) groups at physiological pH (6.5–7.5), forming stable thioether conjugates. The extended hydrophilic spacer minimizes steric hindrance, thereby enhancing the labeling efficiency of proteins, antibodies, peptides, and other biomolecules containing free cysteine residues or thiols introduced through the reduction of disulfide bonds.
The dye exhibits excellent water solubility and low pH sensitivity. It is ideally suited for confocal microscopy, super-resolution microscopy (STED, SIM), and long-duration experiments due to its high photobleaching resistance.
Absorptions- und Emissionsspektren von AF 594
Empfohlenes Protokoll
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General properties
| Appearance: | dark blue crystals |
| Molecular weight: | 887.00 |
| Molecular formula: | C44H46N4O12S2 |
| IUPAC name: | 4-[5-(2,5-dioxopyrrol-1-yl)pentylcarbamoyl]-2-[6,7,7,19,19,20-hexamethyl-9,17-bis(sulfomethyl)-2-oxa-20-aza-6-azoniapentacyclo[12.8.0.03,12.05,10.016,21]docosa-1(14),3,5,8,10,12,15,17,21-nonaen-13-yl]benzoic acid |
| Solubility: | good solubility in water, DMSO, DMF, and acetonitrile; limited solubility in DCM |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| Sicherheitsdatenblatt: | herunterladen |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 586 |
| ε, L⋅mol−1⋅cm−1: | 105000 |
| Emission maximum, nm: | 613 |
| Fluorescence quantum yield: | 0.77 |
| CF260: | 0.28 |
| CF280: | 0.51 |
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