Universal CPG type II, 1400A
| Artikel-Nr. | Packungseinheit | Preis | Vorlaufzeit | Jetzt kaufen |
|---|---|---|---|---|
| 5419-1g | 1 g |
98 €
|
12 Tagen. |
The Universal CPG (Controlled Pore Glass) type II, 1400 Å, is a universal support used to immobilize nucleosides for synthesizing oligonucleotides and to increase the rate of dephosphorylation of the 3′ end oligonucleotide during deblocking.
For the cleavage from the support and oligonucleotide deprotection, anhydrous ammonia gas-phase, ammonium hydroxide/methylamine mixture (AMA), and other basic reagents can be used in a short time. The Universal CPG type II 1400A is suitable for use in harsh conditions and makes cleavage and deprotection faster compared to universal supports. A pore size of 1400 Å is recommended for the synthesis of oligonucleotides 120-180 base pairs in length. For shorter oligos, use universal support universal support 500 Å and 1000 Å.
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General properties
| Appearance: | white powder |
| Quality control: | loading measurement, functional testing in oligo synthesis |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| Sicherheitsdatenblatt: | herunterladen |
| Product specifications |
Oligo synthesis details
| Pore size, Å: | 1400 |
| Typical loading, umol/g: | 40 |
| Coupling conditions: | standard conditions for universal CPG |
| Cleavage conditions: | 2 hours at 80 °C or 8 hours at 55 °C using concentrated ammonia; 15 minutes at 65 °C using AMA mixture, ammonium hydroxide - 40% methylamine (1:1) |
| Deprotection conditions: | identical to protected nucleobases |
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