sulfo-Cyanine5 TCO
| Artikel-Nr. | Packungseinheit | Preis | Vorlaufzeit | Jetzt kaufen |
|---|---|---|---|---|
| 133H0 | 1 mg |
$290.00
|
Auf Lager | |
| 233H0 | 5 mg |
$348.00
|
Auf Lager | |
| 333H0 | 10 mg |
$578.00
|
Auf Lager | |
| 433H0 | 25 mg |
$1220.00
|
Auf Lager |
sulfo-Cyanine5 TCO is a water-soluble far-red fluorescent dye derived from sulfo-Cyanine5 and functionalized with a trans-cyclooctene (TCO) group. The molecule is designed for rapid and highly selective bioorthogonal labeling of tetrazine-containing biomolecules via the inverse electron-demand Diels—Alder (IEDDA) reaction.
The sulfo-Cyanine5 fluorophore belongs to the family of cyanine dyes emitting in the far-red region of the spectrum and is characterized by high molar absorptivity, a good quantum yield, and excellent photostability. The presence of two sulfonate groups provides high water solubility and reduces nonspecific interactions with biomolecules, making the dye well-suited for biological applications.
The trans-cyclooctene moiety enables exceptionally fast reaction kinetics with tetrazines, allowing efficient labeling at low reagent concentrations and under mild conditions, including experiments with live cells.
Absorptions- und Emissionsspektren von Sulfo-Cyanin5
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General properties
| Appearance: | deep-blue crystals |
| Molecular weight: | 931.27 |
| Molecular formula: | C47H63KN4O9S2 |
| Solubility: | water, DMSO, DMF |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| Sicherheitsdatenblatt: | herunterladen |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 271000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |
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