ProbeMaster® Lyo UDG, 5×
| Artikel-Nr. | Packungseinheit | Preis | Vorlaufzeit | Jetzt kaufen |
|---|---|---|---|---|
| L0514 | 100 rxn |
$119.00
|
20 Tagen. |
ProbeMaster® Lyo UDG is a lyophilized reaction mixture containing all the necessary components for PCR. To reconstitute the mixture into a liquid form, simply add the specified amount of water. The composition of the mixture includes Hot-start polymerase and is optimized to achieve optimal amplification processivity and specificity. Uracil-DNA glycosylase eliminates contamination from amplicons generated in prior reactions, preventing false-positive results.
The ProbeMaster® Lyo UDG mixture is suitable for both quantitative PCR and DNA amplification followed by electrophoresis detection. For qPCR reactions, fluorescence detection should use a DNA probe labeled with a fluorophore and quencher (hydrolyzable probes, molecular "beacons", "scorpion" primers) or two probes labeled with fluorophores that form a FRET pair. In addition to DNA probes, the intercalating dye dsGreen can be used for fluorescence detection.
One tube of lyophilized mixture, after dilution in 450 μL of water, is sufficient for 100 reactions of 25 μL each.
Reaction mixture composition
- HS Taq DNA polymerase;
- Uracil-DNA glycosylase (UDG);
- Deoxynucleoside triphosphate mixture (including dUTP);
- PCR buffer (contains Mg2+ at a concentration of 3 mM in 1× reaction mixture);
- Cryoprotectants
Applications
Qualitative and quantitative PCR with detection of amplification products both in real-time and using gel electrophoresis; reverse transcription PCR.
For cloning and other applications requiring further work with the PCR product after amplification, we recommend using our ProbeMaster® Lyo UNI product, which does not contain dUTP.
Equipment compatibility
Compatible with all types of thermal cyclers.
PCR reaction mixture selection table
| Reaction mixtures for quantitative PCR (RT-PCR) | |||||
|---|---|---|---|---|---|
| Name | dsGreen | Eva488 | ROX | UDG, dUTP | Application |
| ProbeMaster®️ Lyo UDG Cat.# •0514 | — | — | — | ✔ | qPCR with DNA probes or intercalating dye |
| ProbeMaster® Lyo ROX Cat.# •0114 | — | — | ✔ | — | |
| ProbeMaster®️ Lyo Eva488 Cat.# •0614 | — | ✔ | — | — | |
| ProbeMaster®️ Lyo Eva488 ROXCat.# •0714 | — | ✔ | ✔ | — | |
| ProbeMaster® Lyo dsGreen Cat.# •0814 | ✔ | — | — | — | |
| Reaction mixture for standard PCR | |||||
| ProbeMaster® Lyo GEL Cat.# •0024 | — | — | — | — | PCR followed by gel electrophoresis analysis, contains dye for application to gel |
| ProbeMaster® Lyo GEL UDGCat.# •0524 | — | — | — | ✔ | |
| Universal reaction mixture | |||||
| ProbeMaster®️ Lyo UNI Cat.# •0534 | — | — | — | — | qPCR with DNA probes/intercalating dye or standard PCR followed by gel electrophoresis analysis |
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General properties
| Appearance: | white tablet |
| Solubility: | water |
| Quality control: | functional test |
| Storage conditions: |
Storage: 12 months (from the date of delivery) at 4 °C. Transportation: Up to 21 days at temperatures up to 25 °C. After reconstitution into liquid form, store at 4 °C for up to 30 days or freeze and store at −20 °C within the shelf life. The reconstituted mixture may undergo up to five freeze–thaw cycles. |
| Sicherheitsdatenblatt: | herunterladen |
| Product specifications |
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