LumiTracker Lyso Green
|A1201||1 x 50 uL||–||Auf Lager|
|11201||20 x 50 uL||$340.00||Auf Lager|
LumiTracker Lyso Green is a dye that can freely permeate through the cell membranes without any additional detergents; it concentrates in organelles with a low pH such as lysosomes. In contrast with 2,4-dinitrophenol (DNP), this dye does not require the use of antibodies for organelle visualization.
The LumiTracker Lyso Green molecule contains boron dipyrromethene (BDP) fragment, which gives it hydrophobic properties and good cell membrane permeability. In addition, this dye contains an amino group that acts as a weak base. At neutral pH, it is not completely protonated, and the dye molecule remains relatively neutral, thus allowing better cell membrane permeability. Getting into lysosomes with an acidic pH, the dye is likely to become protonated and thus remains in the organelle with a low pH.
Living cells are labeled with nanomolar concentrations of LumiTracker Lyso Green, and staining runs within several minutes. LumiTracker Lyso Green can alter lysosomal pH, so longer incubation can result in increased pH, and it is recommended to stain for 3 to 7 min at 37°C (depending on the specific cell line and staining protocol) before visualization using fluorescent microscopy.
LumiTracker Lyso Green has an absorption maximum at 504 nm and a fluorescence maximum at 511 nm. We also offer LumiTracker Lyso Red for organelle visualization in the red channel (absorption and fluorescence maxima at 577 nm and 590 nm, respectively).
LumiTracker Lyso Green is supplied as a 1 mM solution in DMSO.
Recommendations for using the dye:
Working concentration: 50 to 75 nM.*
Staining: 3 to 7 min at 37°C in 1× PBS (just before visualization, as a separate stage during combined staining with other dyes).
*Concentration and/or staining time can be increased depending on the specific cell line.
LysoTracker™ is a registered trademark of Thermo Fisher Scientific in some countries.
|Lagerungsbedingungen:||12 Monate nach Wareneingang bei −20 °C im Dunkeln. Transport: bei Raumtemperatur bis zu drei Wochen. Trocken lagern. Längere Lichteinwirkung und wiederholte Frier-Tau-Zyklen vermeiden. Den Farbstoff nach Möglichkeit aliquotieren.|