Cyanine5.5 phosphoramidite
Artikel-Nr. | Packungseinheit | Preis | Vorlaufzeit | Jetzt kaufen |
---|---|---|---|---|
2680-100mg | 100 mg | – | Auf Lager | |
2680-250mg | 250 mg | $290 | Auf Lager | |
2680-1g | 1 g | $950 | Auf Lager | |
2680-10g | 10 g | auf Anfrage | 5 Tagen. |
Cyanine5.5 is a fluorophore with the emission in far-red range of the spectrum. This dye is useful for multiplex qPCR. Commercial six-channel qPCR instruments often have a channel for Cyanine5.5.
This phosphoramidite can be used for the synthesis of 5'-labeled oligonucleotides by direct labeling in oligonucleotide synthesizer.
The structure of phosphoramidite functional group that is attached to a secondary carbon atom provides extra stability against Arbuzov rearrangement. This helps maintaining coupling performance over a longer storage time in oligonucleotide synthesizer, compared to phosphoramidites derived of primary alcohols.
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Inosin (dI) Phosphoramidit
Dimethoxytrityl-2'-Desoxyinosin (DMT-dI) ist ein Phosphoramidit für die Synthese inosinhaltiger DNA-Oligonukleotide.DusQ 2 CPG 500
Ein CPG 500-Trägermaterial zur Synthese von Oligonukleotiden mit DusQ 2-Quencher am 3’-Ende. Es ist mit Standardbedingungen zum Entschützen von Oligonukleotiden kompatibel.dsGreen für Echtzeit-PCR, 100×
Fluoreszierender Farbstoff zum Nachweis der Amplifikation in der Echtzeit-PCR in einer gebrauchsfertigen 100× Stammlösung zur Zugabe zum Mastermix.General properties
Appearance: | dark colored solid |
Molecular weight: | 916.61 |
Molecular formula: | C55H71N5ClO3P |
Quality control: | NMR 1H, 31P, HPLC-MS (85%) |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Sicherheitsdatenblatt: | herunterladen |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 694 |
ε, L⋅mol−1⋅cm−1: | 198000 |
Emission maximum, nm: | 710 |
Fluorescence quantum yield: | 0.2 |
CF260: | 0.07 |
CF280: | 0.03 |
Oligo synthesis details
Diluent: | acetonitrile |
Coupling conditions: | 6 min coupling time recommended |
Deprotection conditions: | recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible |