Keep fluorescence with our mounting medium
The final step of fluorescent staining of tissues and cells is their mounting under coverslips. The quality of microscope images depends on the choice of mounting medium. The medium must preserve the fluorescent signal, protect the sample from drying out and degradation, have a low viscosity for uniform distribution throughout the sample, and be optically similar to the coverslip.
Selecting a mounting medium with a refractive index close to 1.52 is essential to reduce light reflection and ensure maximum transparency and image quality. Moreover, for fluorescent dyes, such a medium must be prepared on an aqueous basis.
We offer mounting media that have all of the above characteristics. Our media will help preserve sample fluorescence and provide optimal conditions for sample storage and analysis.
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LumiMountLumiMount is an aqueous-based mounting medium for coverslipping cells and tissues stained with various fluorescent dyes. LumiMount reduces fluorochrome quenching during imaging due to anti-fade agent content. The refractive index of a liquid mounting medium is 1.38, but it can increase to 1.46 as the water evaporates at the edges of the coverslip. |
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LumiMount DAPILumiMount DAPI is an aqueous-based mounting medium with DAPI, a blue-emitting fluorescent dye that binds strongly to DNA and is used as a nuclear counterstain. The refractive index of a liquid mounting medium is 1.38, but it can increase to 1.46 as the water evaporates at the edges of the coverslip. |
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LumiMount PlusLumiMount Plus is a ready-to-use aqueous-based mounting medium for coverslipping and imaging fluorescently labeled cell or tissue samples. LumiMount Plus is more viscous than LumiMount, which makes it less prone to bubbles. The refractive index of liquid LumiMount Plus is 1.43, but this property increases up to 1.52 after water evaporation and solidification of mounting. The high refractive properties of the medium make it promising for use in super-resolution microscopy. LumiMount Plus is not compatible with intercalating dyes such as Hoechst, DAPI, etc. |
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