QuDye Protein Quantification Kit
| Artikel-Nr. | Packungseinheit | Preis | Vorlaufzeit | Jetzt kaufen |
|---|---|---|---|---|
| A5102 |
30 assays
|
– | 1 Tagen. | |
| 15102 |
100 assays
|
$110 | 1 Tagen. | |
| 16102 |
100 assays (incl. tubes)
|
$130 | 1 Tagen. | |
| 25102 |
500 assays
|
$350 | 1 Tagen. |
The kit includes concentrated 200x assay reagent, dilution buffer, and protein standards 0, 200, and 400 ng/uL optimized for the area of measurements. The assay has a low variability depending on the protein type and is tolerant to contaminants, such as reducing agents, nucleotides, and free amino acids DNA, but not detergents. This kit allows accurate protein quantification in the range from 12.5 µg/mL to 5 mg/mL for the initial sample (protein amount range for measuring using fluorometer: 0.25-5 µg of protein in 200 µL of the test sample). The experiment is performed at room temperature, and the signal is stable for 3 hours.
The quality of measurements can be even higher when the same buffer is used to calculate the background and to prepare the sample.
This kit allows simple diluting of the reagent concentrate and starting the measurements. Fluorometer instrument allows to read samples with volume from 1–20 uL.
Allgemeine Eigenschaften
| Lagerungsbedingungen: | Bei +4 °С lagern. Reagenzien vor Gebrauch auf +20 °C temperieren. |
| Sicherheitsdatenblatt: | herunterladen |
Kit
| Lagerzeit in Monaten: | 12 |
Referenzen
- Shishkova, D.; Lobov, A.; Repkin, E.; Markova, V.; Markova, Y.; Sinitskaya, A.; Sinitsky, M.; Kondratiev, E.; Torgunakova, E.; Kutikhin, A. Calciprotein Particles Induce Cellular Compartment-Specific Proteome Alterations in Human Arterial Endothelial Cells. Journal of Cardiovascular Development and Disease, 2024, 11(1), 5. doi: 10.3390/jcdd11010005
- Sinitsky, M.; Repkin, E.; Sinitskaya, A.; Markova, V.; Shishkova, D.; Barbarash, O. Proteomic Profiling of Endothelial Cells Exposed to Mitomycin C: Key Proteins and Pathways Underlying Genotoxic Stress-Induced Endothelial Dysfunction. International Journal of Molecular Sciences, 2024, 25(7), 4044. doi: 10.3390/ijms25074044
- Frolov, A.; Lobov, A.; Kabilov, M.; Zainullina, B.; Tupikin, A.; Shishkova, D.; Markova, V.; Sinitskaya, A.; Grigoriev, E.; Markova, Y.; Kutikhin, A. Multi-Omics Profiling of Human Endothelial Cells from the Coronary Artery and Internal Thoracic Artery Reveals Molecular but Not Functional Heterogeneity. International Journal of Molecular Sciences, 2023, 24(19), 15032. doi: 10.3390/ijms241915032
- Semenova, D.; Zabirnyk, A.; Lobov, A.; Boyarskaya, N.; Kachanova, O.; Uspensky, V.; Zainullina, B.; Denisov, E.; Gerashchenko, T.; Kvitting, J.-P. E.; Kaljusto, M.-L.; Thiede, B.; Kostareva, A.; Stensløkken, K.-O.; Vaage, J.; Malashicheva, A. Multi-Omics of in Vitro Aortic Valve Calcification. Frontiers in Cardiovascular Medicine, 2022, 9, 1043165. doi: 10.3389/fcvm.2022.1043165
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