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81

What does "minimal" mean in Cyanine NHS ester minimal dye products, and can Cyanine2, Cyanine3, and Cyanine5 be mixed all together?


Help "Minimal" meaning in Cyanine NHS ester minimal dye products for DIGE applications


82

How much of dye is in each reaction sulfo-Cyanine3 antibody labeling kit?


Help Amount of dye per reaction in sulfo-Cyanine3 antibody labeling kit


83

Does the fluorescence of Cyanine dyes depend on pH?


Help The fluorescence dependency of Cyanine dyes such as Cyanine3, sulfo-Cyanine3, Cyanine5, sulfo-Cyanine5, and boron-dipyrromethene dyes such as BDP TMR, BDP 630/650 on pH conditions.


84

How to use your fluorescent dyes in proteomics applications like difference gel electrophoresis (DIGE)?


Help Protein labeling with Cyanine2, Cyanine3, and Cyanine5 minimal dyes for 2D DIGE Electrophoresis.


85

Any suggestions for FRET pair of dyes?


Help FRET pairs of dyes we can recommend from our catalog products


86

How to attach DBCO (ADIBO) group to an antibody?


Help Description of a short experiment we have performed to introduce DBCO label into a mouse monoclonal antibody.


87

Does azide ion interfere with click chemistry reactions (CuAAC and SPAAC)?


Help Decreased labeling efficiency of CuAAC and SPAAC click reactions due to azide presence in reaction buffer.


88

Does nonspecific protein labeling exist in click chemistry reactions?


Help A comparison of nonspecific interactions in different click chemistry reactions, namely Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted alkyne-azide cycloaddition (SPAAC).


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